Journal: Breast Cancer : Targets and Therapy

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells

doi: 10.2147/BCTT.S85202

Figure Lengend Snippet: Gene expression among different cancer types. Notes: Relative gene expression analysis of nine genes that were overexpressed in breast cancer and breast CSCs. The gene expression also was studied in colon and lung cancer, colon CSCs, lung CSCs, embryonic stem cells, and in a reference sample. The experiments were performed in triplicate and a P -value of <0.05 was considered significant. Abbreviations: CSCs, cancer stem cells; ESCs, embryonic stem cells; HP , haptoglobin; SMPD1 , sphingomyelin phosphodiesterase 1, acid lysosomal; SCRIB , scribbled homolog ( Drosophila ); KCMF1 , potassium channel modulatory factor 1; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; TMX2 , thioredoxin-related transmembrane protein 2; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Article Snippet: During the exponential phase of proliferation, commercial breast CSCs cells were plated in 24-well plates (E36102-29-24Well; Celprogen) and transfected with gene-specific small interfering RNAs (siRNAs) using Lipofectamine 2000 Reagent (11668-027; Thermo Fisher Scientific), according to the manufacturer’s instructions.

Techniques: Expressing, Sequencing

Journal: Breast Cancer : Targets and Therapy

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells

doi: 10.2147/BCTT.S85202

Figure Lengend Snippet: Breast CSCs pre- and post-siRNA knockdown. Note: Representative images showing breast CSCs pre- and post-siRNA knockdown. Abbreviations: CSCs, cancer stem cells; siRNA, small interfering RNA; TMX2 , thioredoxin-related transmembrane protein 2; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49.

Article Snippet: During the exponential phase of proliferation, commercial breast CSCs cells were plated in 24-well plates (E36102-29-24Well; Celprogen) and transfected with gene-specific small interfering RNAs (siRNAs) using Lipofectamine 2000 Reagent (11668-027; Thermo Fisher Scientific), according to the manufacturer’s instructions.

Techniques: Small Interfering RNA, Sequencing

Journal: Breast Cancer : Targets and Therapy

Article Title: Identification of genes involved in breast cancer and breast cancer stem cells

doi: 10.2147/BCTT.S85202

Figure Lengend Snippet: Gene expression of stemness transcription factors in breast CSCs. Notes: Relative gene expression of transcription factors in breast CSCs following knockdown. The ∆∆Ct method was used to perform the analysis. Each bar represents the average of the Ct values. The assays were performed in triplicate and a P -value of <0.05 was considered to be significant. The assays are presented in a log2 scale. Thus, positive values indicate overexpression while negative values indicate underexpression. Abbreviations: CSCs, cancer stem cells; TMX2 , thioredoxin-related transmembrane protein 2; FAM155B , family with sequence similarity 155, member B; PTGER3 , prostaglandin E receptor 3 (subtype EP3); GPR3 , G protein-coupled receptor 3; DDX49 , DEAD (Asp-Glu-Ala-Asp) box polypeptide 49; NANOG , Homeobox protein NANOG; OCT3/4 , Octamer-binding transcription factor ¾; SOX2 , sex determining region Y-box 2; CD34 , Hematopoietic progenitor cell antigen CD34.

Article Snippet: During the exponential phase of proliferation, commercial breast CSCs cells were plated in 24-well plates (E36102-29-24Well; Celprogen) and transfected with gene-specific small interfering RNAs (siRNAs) using Lipofectamine 2000 Reagent (11668-027; Thermo Fisher Scientific), according to the manufacturer’s instructions.

Techniques: Expressing, Over Expression, Sequencing, Binding Assay

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the migration of HepG2 cells in a scratch test. The scratch test was used to investigate the wound healing ability at 0, 12, 24 and 48 h. We observed that the proliferation and migration ability of HepG2 cells in the wounded area was reduced in the miR-1301 group after a 24 and 48 h repair period when compared with the control group. There was no difference between the miR-1301 transfected and the control group at 12 h.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Migration, Transfection

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits the invasion of HepG2 cells in the Transwell chamber assay. Cell invasion ability was analyzed by the Transwell chamber assay 48 h after miR-1301 transfection. The results showed that the number of migration cells in the miR-1301 group was less than that in control group. This indicated that the invasion ability of HepG2 cells might be inhibited by miR-1301.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Transwell Chamber Assay, Transfection, Migration

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 inhibits cell proliferation. The MTT assay was performed to monitor the proliferation rate of HepG2 cells after miR-1301 transfection. The optical density of each well was measured with a microplate spectrophotometer at 490 nm. The optical density of the miR-1301 group decreased at 24 h (A), 48 h (B), and 72 h (C) after transfection. The proliferation rate of cells decreased with increasing concentrations of inhibitor and increasing transfection time (D).

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: MTT Assay, Transfection, Spectrophotometry

Journal: Oncology Reports

Article Title: microRNA-1301-mediated inhibition of tumorigenesis

doi: 10.3892/or.2011.1589

Figure Lengend Snippet: miR-1301 promotes cell apoptosis. Apoptosis of HepG2 cells was observed using fluorescence microscopy through a dual pass filter allowing to visualize the Annexin-V-FITC positive and the propidium iodide positive cells in the same field. The results showed that apoptosis of HepG2 cells increased 48 h after transfection of miR-1301 mimics in the miR-1301 group (Aa) when compared with the control group (Ba). (Aa) Apoptosis cells of the Annexin-V-FITC positive cells in the miR-1301 group; (Ab) cells excited by normal light in the miR-1301 group; (Ba) apoptosis cells of the Annexin-V-FITC positive cells in the control group; (Bb) cells excited by normal light in the control group.

Article Snippet: Total RNA was extracted from human HepG2 cell lines using TRIzol reagent (Takara Biotechnology Co., Ltd.).

Techniques: Fluorescence, Microscopy, Transfection

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. Light field of SSCs cultured with melatonin and GDNF, bar=50 μm. B. Cell density after being cultured with different cell mediums at 48 h. The initial number was 5*10 4 . C. QRT-PCR and western blot analysis of proliferation, self-renewal and Sertoli cell markers. D. Western blot analysis of proliferation and Sertoli cell markers. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Cell Culture, Quantitative RT-PCR, Western Blot

Journal: Oncotarget

Article Title: Melatonin promotes goat spermatogonia stem cells (SSCs) proliferation by stimulating glial cell line-derived neurotrophic factor (GDNF) production in Sertoli cells

doi: 10.18632/oncotarget.12720

Figure Lengend Snippet: A. ELISA analysis of GDNF levels in the SSCs medium. B. Western Blot analysis of phosphorylation levels of AKT and ERK. *, P<0.05,**, P<0.01.

Article Snippet: GDNF levels were determined by using a Human glial cell line-derived neurotrophic factor (GDNF) ELISA Kit (BOSTER).

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot, Phospho-proteomics